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sera & serum-free media 首頁 Home > StemCell Technologies Inc > sera & serum-free media

products

Serum-Free Media & Related Products
StemSpan SFEM - Serum-Free Expansion Medium
StemSpan nsp;H3000 - Defined Medium
BIT 9500 - Serum Substitute
Bovine Serum Albumin for Human and Mouse Cells
StemSpan CC100 - Cytokine Cocktail
StemSpan CC110 - Cytokine Cocktail
StemSpan CC200 - Cytokine Cocktail

Pre-Screened Sera for Specialized Applications
For Human Myeloid Colony-Forming Cells
For Mouse Myeloid Colony-Forming  Cells
For Human Myeloid Long-Term Culture
For Mouse Myeloid Long-Term Culture
For Mouse Pre-B Lymphoid Colony-Forming Cells
For Mouse B-Lymphoid Long-Term Culture
For Human Mesenchymal Cells
For Human Osteogenic Differentiation
For Mouse Embryonic Stem Cells
For In Vitro Differentiation of Mouse ES Cells - Hematopoietic
For In Vitro Differentiation of Mouse ES Cells - Pancreatic or Neural
Horse Serum for Human Long-Term Culture
Horse Serum for Mouse Long-Term Culture

background

Serum-Free Media. The ability to maintain, expand and assay hematopoietic stem cells and progenitors in vitro has been instrumental in advancing our understanding of hematopoiesis. Historically, medium containing fetal bovine serum (FBS) has been used for culture of hematopoietic cells. However, the concentrations of inhibitors including transforming growth factor-?and other constituents vary between batches of sera. In order to minimize these variables, particularly for studies of the effects of specific regulatory or growth factors, there has been a move towards the use of serum-free media. StemCell Technologies is a leading supplier of serum substitutes and serum-free media that support the maintenance and expansion of hematopoietic stem cells and progenitors. We offer a range of products that includes StemSpanSFEM and StemSpan?H3000, our new animal-free medium containing only human-derived or recombinant human proteins.

Sera. The fetal bovine sera and horse sera used in our culture media are rigorously screened and selected for specific applications. Selection and validation of sera and other components used for in vitro hematopoietic colony assays and long-term cultures are key factors of all our culture reagents. This allows investigators to take advantage of our extensive experience when preparing their own media formulations. StemCell Technologies extensively screens lots of the highest quality fetal bovine and horse sera. Sera is collected according to standards of United States Department of Agriculture.

applications
StemSpan Srum-Free Media

Generation and culture of dendritic cells
Expansion of normal human hematopoietic cells including: clonogenic progenitors1-5, long-term culture-initiating cells (LTC-IC)3-5, in vivo competitive repopulating units (human CRU) from cord blood5,6
Transduction of hematopoietic progenitors by retroviral supernatant infection7
Detection of acute myeloid leukemic (AML) cells with long-term proliferative potential8
Amplification of mouse bone marrow CRU (cells with competitive long-term in vivo repopulating potential)9,10
Generation of B cell progenitors and myeloid cells from mouse Sca1+ Lin- fetal liver cells11
Defined formulation facilitates the identification of novel regulatory factors